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The striking and unexpected cytogenetic diversity of genus Tanacetum L. (Asteraceae): A cytometric and fluorescent in situ hybridisation study of Iranian taxa

机译:Tanacetum L.(Asteraceae)的惊人和出乎意料的细胞遗传学多样性:伊朗类群的细胞计数和荧光原位杂交研究

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摘要

[Background] Although karyologically well studied, the genus Tanacetum (Asteraceae) is poorly known from the perspective of molecular cytogenetics. The prevalence of polyploidy, including odd ploidy warranted an extensive cytogenetic study. We studied several species native to Iran, one of the most important centres of diversity of the genus. We aimed to characterise Tanacetum genomes through fluorochrome banding, fluorescent in situ hybridisation (FISH) of rRNA genes and the assessment of genome size by flow cytometry. We appraise the effect of polyploidy and evaluate the existence of intraspecific variation based on the number and distribution of GC-rich bands and rDNA loci. Finally, we infer ancestral genome size and other cytogenetic traits considering phylogenetic relationships within the genus.
机译:[背景]尽管对人体进行了深入的研究,但从分子细胞遗传学的角度来看,艾菊属(菊科)知之甚少。多倍体的流行,包括奇数倍性,值得进行广泛的细胞遗传学研究。我们研究了几种伊朗最原始的物种,伊朗是该物种最重要的多样性中心之一。我们旨在通过荧光染料条带,rRNA基因的荧光原位杂交(FISH)和通过流式细胞仪评估基因组大小来表征艾菊的基因组。我们评估多倍体的效果,并根据富含GC的条带和rDNA基因座的数量和分布评估种内变异的存在。最后,考虑属内的系统发育关系,我们推断祖先的基因组大小和其他细胞遗传学特征。

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